Search results for "Cyclic RGD"

showing 3 items of 3 documents

Immobilisation of linear and cyclic RGD-peptides on titanium surfaces and their impact on endothelial cell adhesion and proliferation

2011

Functional coatings on titanium vascular stents and endosseous dental implants could probably enhance endothelial cell (EC) adhesion and activity with a shortening of the wound healing time and an increase of peri-implant angiogenesis during early bone formation. Therefore, the role of the structure of linear and cyclic cell adhesive peptides Arg-Gly-Asp (l-RGD and c-RGD) on differently pre-treated titanium (Ti) surfaces (untreated, silanised vs. functionalised with l- and c-RGD peptides) on EC cell coverage and proliferation was evaluated. After 24 h and after 3 d, surface coverage of adherent cells was quantifi ed and an alamarBlue® proliferation assay was conducted. After 24 h, l-RGD mod…

AdultMaleTime Factorslcsh:Diseases of the musculoskeletal systemSurface PropertiesAngiogenesisCelllcsh:Surgerychemistry.chemical_elementCoated Materials BiocompatibleRGD modificationCell AdhesionmedicineHumanstitaniumcyclicCells CulturedCell ProliferationCell growthlcsh:RD1-811AdhesionMolecular biologyendothelial cellsEndothelial stem cellimmobilisationmedicine.anatomical_structurelinearchemistryMicroscopy Electron ScanningFemalelcsh:RC925-935Cyclic RGDWound healingOligopeptidesBiomedical engineeringTitaniumEuropean Cells and Materials
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Photoactivatable caged cyclic RGD peptide for triggering integrin binding and cell adhesion to surfaces.

2011

We report the synthesis and properties of a photoactivatable caged RGD peptide and its application for phototriggering integrin- and cell-binding to surfaces. We analysed in detail 1) the differences in the integrin-binding affinity of the caged and uncaged forms by quartz crystal microbalance (QCM) studies, 2) the efficiency and yield of the photolytic uncaging reaction, 3) the biocompatibility of the photolysis by-products and irradiation conditions, 4) the possibility of site, temporal and density control of integrin-binding and therefore human cell attachment, and 5) the possibility of in situ generation of cell patterns and cell gradients by controlling the UV exposure. These studies p…

In situIntegrinsBiocompatibilityStereochemistryUltraviolet RaysCellIntegrinBiochemistryPeptides CyclicmedicineCell AdhesionHumansCell adhesionMolecular BiologyCells CulturedIntegrin bindingMaterialesbiologyChemistryOrganic ChemistryQuímicaQuartz crystal microbalanceCyclic rgd peptidemedicine.anatomical_structureBiophysicsbiology.proteinQuartz Crystal Microbalance TechniquesMolecular MedicineProtein BindingChembiochem : a European journal of chemical biology
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Blend scaffolds with polyaspartamide/polyester structure fabricated via TIPS and their RGDC functionalization to promote osteoblast adhesion and prol…

2019

Target of this work was to prepare a RGDC functionalized hybrid biomaterial via TIPS technique to achieve a more efficient control of osteoblast adhesion and diffusion on the three-dimensional (3D) scaffolds. Starting from a crystalline poly(l-lactic acid) (PLLA) and an amorphous alpha,beta-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-d,l-aspartamide-graft-polylactic acid (PHEA-EDA-g-PLA) copolymer, blend scaffolds were characterized by an appropriate porosity and pore interconnection. The PHEA-EDA-PLA interpenetration with PLLA improved hydrolytic susceptibility of hybrid scaffolds. The presence of free amino groups on scaffolds allowed to tether the cyclic RGD peptide (RGDC) via Michael…

Materials sciencePolyesters0206 medical engineeringBiomedical EngineeringBiocompatible Materialscyclic RGDC02 engineering and technologyPeptides CyclicPLLACell LineBiomaterialsMiceHydrolysischemistry.chemical_compoundCell AdhesionCopolymerAnimalsCell adhesionMaleimideporous scaffoldCell ProliferationOsteoblastsTissue ScaffoldsMetals and AlloysBiomaterialPHEA021001 nanoscience & nanotechnology020601 biomedical engineeringPolyesterChemical engineeringchemistryCeramics and CompositesMichael reactionSurface modificationTIPSPeptides0210 nano-technology
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